|
| Status |
Public on Dec 17, 2010 |
| Title |
transcriptional profiling of CD133+ cells in coronary artery disease, SG-25A |
| Sample type |
RNA |
| |
|
| Source name |
CAD patient, baseline
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
| Treatment protocol |
Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
|
| Growth protocol |
CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
|
| Scan protocol |
Affymetrix GeneChip scanner.
|
| Description |
CD133+ cells sorted from PB.
SG-25A
|
| Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
|
| |
|
| Submission date |
Oct 16, 2009 |
| Last update date |
Dec 17, 2010 |
| Contact name |
Delong Liu |
| E-mail(s) |
liud2@mail.nih.gov
|
| Phone |
301-451-7129
|
| Organization name |
NHLBI/NIH
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18608 |
Transcriptional Profiling of CD133+ Cells in Coronary Artery Disease and Effects of Exercise on Gene Expression |
|
