|
| Status |
Public on Nov 29, 2020 |
| Title |
ChIP-seq of H3K4me3 in atx1-II |
| Sample type |
SRA |
| |
|
| Source name |
inflorescence stems
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: inflorescence stems
chip antibody: H3K4me3 (Millipore, 04-745)
genotype: atx1
stem region: II
|
| Growth protocol |
Arabidopsis were grown under long day (16h light, 8h dark, 22℃)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Histone-DNA complexes were isolated with H3K4me3 antibody
The ChIP-seq library was constructed by Novogene Corporation (Beijing, China).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
ChIP-seq reads were aligned to Arabidopsis thaliana genome (TAIR 10) using BWA with default parameters and only uniquely mapped reads were kept.
Then MACS2 (version 2.1.0) peak calling software was used to identify regions of IP enrichment over background.
Genome_build: TAIR10
Supplementary_files_format_and_content: bigwig
|
| |
|
| Submission date |
Jul 02, 2020 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Xianqiang Wang |
| E-mail(s) |
Xianqiang_Wang@hotmail.com
|
| Organization name |
Southwest University
|
| Street address |
Tiansheng Road
|
| City |
Chongqing |
| ZIP/Postal code |
400715 |
| Country |
China |
| |
|
| Platform ID |
GPL26208 |
| Series (1) |
| GSE153705 |
Histone H3K4 methyltransferase ATX1 positively regulates secondary wall biosynthesis in interfascicular fibers in Arabidopsis |
|
| Relations |
| BioSample |
SAMN15425043 |
| SRA |
SRX8657074 |
