|
| Status |
Public on Oct 27, 2009 |
| Title |
RZ-5 |
| Sample type |
RNA |
| |
|
| Source name |
growth plate chondrocytes, reserve zone
|
| Organism |
Bos taurus |
| Characteristics |
cell type: growth plate chondrocytes, reserve zone
age: < 10 months
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using RNA STAT-60 (Tel-Test, Inc.) per the manufacturer’s instructions. Samples were analyzed with an Agilent 2100 Bioanalyzer to confirm RNA integrity.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the MessageAmp II-Biotin Enhanced Kit from Ambion
|
| |
|
| Hybridization protocol |
Following fragmentation, hybridization was performed according to standard Affymetrix (Santa Clara, CA) protocols (http://www.affymetrix.com/support/technical/manuals/affx).
|
| Scan protocol |
GeneChips were scanned at high resolution using an Affymetrix GeneChip Scanner 3000.
|
| Description |
RZ-5
|
| Data processing |
Results were analyzed using GeneSpring GX version 7.2 software (Agilent Technologies, Redwood City, CA). Data, chip and genes were normalized in three stages (log transformed raw data < .01 set to .01, global normalization to the 50th percentile, followed by gene level normalization) using the recommended GeneSpring default settings for data transformation.
|
| |
|
| Submission date |
Oct 26, 2009 |
| Last update date |
Oct 26, 2009 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
sochsner@bcm.edu
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE18738 |
Bovine Hypertrophic Growth Plate Chondrocytes vs. Reserve Zone Chondrocytes |
|
