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| Status |
Public on Oct 27, 2009 |
| Title |
Bovine Hypertrophic Growth Plate Chondrocytes vs. Reserve Zone Chondrocytes |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
|
| Summary |
Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. 6 pairs of HZ vs RZ were compared by microarray.
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| Overall design |
Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. Six independent sample pairs of HZ vs RZ were compared by microarray.
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| Contributor(s) |
Hutchison MR, Bassett MH, White PC |
| Citation(s) |
19897599 |
| Submission date |
Oct 26, 2009 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
sochsner@bcm.edu
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA121647 |
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