|
| Status |
Public on Jul 28, 2020 |
| Title |
T1-21 |
| Sample type |
RNA |
| |
|
| Source name |
Renal Cell Carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
metastatic: Yes
tumor stage: 1
gender: F
tissue: Renal Cell Carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RecoverAllTM Total Nucleic Acid Isolation Kit according to manufacturer’s instructions
|
| Label |
TaqMan
|
| Label protocol |
Quantification of miRNA was done using the TaqMan OpenArray, which profiles 754 known miRNAs using qPCR
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
microRNA
|
| Data processing |
A Ct threshold of 30 was used for detectable level of expression.
MiRNAs were further filtered by removing those with no expression in any samples and samples were filtered that had < 10% of miRNAs detected.
Housekeeping miRNAs were the top 10 most stable miRNAs from the NormqPCR package_x0001_, which were used to normalize raw Ct values using ∆Ct normalization
Differential expression was calculated using limma
Matrix normalized worksheet reports normalized signal (against 10 most stable housekeeping genes).
|
| |
|
| Submission date |
Jul 27, 2020 |
| Last update date |
Jul 28, 2020 |
| Contact name |
Andrew Shih |
| E-mail(s) |
ashih@northwell.edu
|
| Phone |
516-562-2960
|
| Organization name |
Feinstein Institute - Northwell Health
|
| Street address |
350 Community Dirive
|
| City |
Mahasset |
| State/province |
NY |
| ZIP/Postal code |
11030 |
| Country |
USA |
| |
|
| Platform ID |
GPL18942 |
| Series (2) |
| GSE155209 |
Prognostic Molecular Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas |
| GSE155210 |
Prognostic Molecular Signatures and Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas |
|
