|
Status |
Public on May 31, 2021 |
Title |
Mdmx C462A no treatment rep1 |
Sample type |
RNA |
|
|
Source name |
MEFs
|
Organism |
Mus musculus |
Characteristics |
cell type: MEFs genotype: Mdmx C462A
|
Treatment protocol |
Contained within publication; detailed protocols are available upon request
|
Growth protocol |
Contained within publication; detailed protocols are available upon request
|
Extracted molecule |
total RNA |
Extraction protocol |
Contained within publication; detailed protocols are available upon request
|
Label |
biotin
|
Label protocol |
Contained within publication; detailed protocols are available upon request
|
|
|
Hybridization protocol |
Contained within publication; detailed protocols are available upon request
|
Scan protocol |
Contained within publication; detailed protocols are available upon request
|
Data processing |
The resultant dataset from the GeneChip™ Mouse Gene 2.0 array was performed in the Transcriptome Analysis Console (v4.0). This software was used for the creation of scatterplots, volcano plots, and hierarchical clustering. From these plots, the threshold for log fold change in gene expression was set at ± 2.0, with statistical significance cutoffs at or below a p-value of 0.05. Using these differentially expressed genes, the software MetaCore+MetaDrug™ (v20.2; build 70100) was used to perform pathway enrichment (PE) as well as gene ontology (GO) analyses (all p-values used were ≤ 0.05). Microsoft Excel was used to transform these results into 1/FDR (false discovery rate) as well as to create the combined figure.
|
|
|
Submission date |
Aug 04, 2020 |
Last update date |
May 31, 2021 |
Contact name |
Zhi-Min Yuan |
Organization name |
Harvard University
|
Department |
Environmental Sciences
|
Street address |
677 Huntington Ave.
|
City |
Boston |
State/province |
Massachusetts |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (2) |
GSE155694 |
Microarray of MDMX C462A and wildtype mice treated with acetaminophen |
GSE155696 |
Hepatic p53 protein level governs liver s sensitivity to drug-induced liver injury |
|