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Status |
Public on Oct 11, 2021 |
Title |
Stabilin-double-deficient LSECs, replicate 3 |
Sample type |
RNA |
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Source name |
C57BL/6J LSECs
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J genotype: Stab DKO cell type: liver sinusoidal endothelial cells (LSECs)
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Treatment protocol |
Murine LSECs from different groups were isolated and purified using CD146 (LSEC) MicroBeads. Purity was assessed using FACS. Three mice per genotype were pooled per biological replicate in Microarray analyis.
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Growth protocol |
RNA was extracted from primary murine LSECs immediately after isolation
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from primary endothelial cells using E.Z.N.A. TM Total-RNA-Kit I (OMEGA Biotec, Norcross, GA, USA).
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Label |
Biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
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Scan protocol |
Affymetrix GeneArray Scanner3000
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Description |
Gene expression data from Stabilin-double-deficient LSECs
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Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
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Submission date |
Sep 14, 2020 |
Last update date |
Oct 11, 2021 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
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Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
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City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
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Platform ID |
GPL20710 |
Series (1) |
GSE157900 |
Analysis of Scavenger-Receptor Type H deficient liver sinusoidal endothelial cells |
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