|
| Status |
Public on Sep 14, 2023 |
| Title |
MED1-KO 3T3-L1 Day2 pool rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MED1-KO 3T3-L1 cells before hormonal stimulation
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Cell line: MED1-KO 3T3-L1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA samples were processed and prepared for whole transcriptome expression analysis using the GeneChip WT PLUS Reagent Kit (Thermofisher 902280)
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45 degrees celcius for 16 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
|
| Scan protocol |
The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from undifferenriated MED1-KO 3T3-L1
|
| Data processing |
The data expressed as CEL files were normalized using the Signal Space Transformation-Robust Multi-Chip Analysis (SST-RMA) algorithm with Transcriptome Analysis Console (TAC) 4.0 (Thermofisher)
|
| |
|
| Submission date |
Sep 16, 2020 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Keiichi Ito |
| E-mail(s) |
keiichiito0918@gmail.com
|
| Phone |
212-327-7605
|
| Organization name |
The Rockefeller University
|
| Lab |
Laboratory of Biochemistry andMolecular Biology
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE158083 |
Gene expression data from differentiating 3T3-L1 cells |
|
