|
| Status |
Public on Jul 14, 2021 |
| Title |
40℃ high tempeature stress condition P169A rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Early-log phase cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
phenotype: Stress tolerant
culture conditions: 40 degrees C high tempeature stress condition
strain: P169A
growth phase: early log
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample were grown on YP medium containing 200 g/l glucose at 30ºC at 200 rpm for 10 h to the early-log phase, and harvested in Falcon tubes precooled in liquid N2 by centrifuging for 5 minutes at 4000 rpm (3220 × g). The supernatant was discarded, and the pellet was stored at −80°C until further use. Total RNA extraction, library construction and sequencing were conducted by Novogene Bioinformatics Technology Co., Ltd (Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
RSEM_LYF18
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, then mapped to reference genome using Bowtie2 with default parametres
Abundance measurements by RSEM
Differential gene expression by R package DESeq2, a p-value cutoff of 0.05 or less and an absolute fold-change threshold of 2.0 or greater.
Genome_build: R64
Supplementary_files_format_and_content: gene expression Raw counts file gene_Raw_counts.txt: text file include all expression gene list with Raw counts
|
| |
|
| Submission date |
Oct 27, 2020 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Qinhong Wang |
| E-mail(s) |
wang_qh@tib.cas.cn
|
| Organization name |
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
|
| Lab |
Wang lab
|
| Street address |
32 West 7th Avenue, Tianjin Airport Economic Area
|
| City |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
| |
|
| Platform ID |
GPL27812 |
| Series (1) |
| GSE160256 |
Base editing of general transcription factor Spt15 to enhance yeast stress tolerance |
|
| Relations |
| BioSample |
SAMN16575638 |
| SRA |
SRX9381189 |
