|
| Status |
Public on Feb 01, 2021 |
| Title |
PfAP2-G2::KO gametocytes TP8 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
PfAP2-G2::KO gametocytes stage III
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: E5
genotype: PfAP2-G2::KO
hours post invasion: Day 4 gametocytes
Stage: stage III
|
| Treatment protocol |
The early gametocyte rings were treated with media containing heparin (20 U/ml) for four days to prevent the reinvasion of asexual parasites.
|
| Growth protocol |
Both strains of Plasmodium falciparum parasites were cultured at 37 deg C under standard conditions in the presence of 5% CO2 and 6% O2 in RPMI 1640 media. The media was supplemented with HEPES, NaHCO3, hypoxanthine, 2.5% (w/v) Albumax II, and gentamycin (50 ug/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from induced PfAP2-G2::KO and WT parasites every 12 hours for 7.5 days using TRIzol. Timepoint 6 was excluded due to collection error.
|
| Label |
Cy5
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinßs, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213û219 (2013).
|
| |
|
| Channel 2 |
| Source name |
3D7 Asexual and Sexual Mixed Stage Reference Pool + cDNA from Gametocyte Samples
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: 3D7 + E5
genotype: PfAP2-G2::KO
Stage: Asexual and Sexual Mixed Stage
|
| Treatment protocol |
The early gametocyte rings were treated with media containing heparin (20 U/ml) for four days to prevent the reinvasion of asexual parasites.
|
| Growth protocol |
Both strains of Plasmodium falciparum parasites were cultured at 37 deg C under standard conditions in the presence of 5% CO2 and 6% O2 in RPMI 1640 media. The media was supplemented with HEPES, NaHCO3, hypoxanthine, 2.5% (w/v) Albumax II, and gentamycin (50 ug/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from induced PfAP2-G2::KO and WT parasites every 12 hours for 7.5 days using TRIzol. Timepoint 6 was excluded due to collection error.
|
| Label |
Cy3
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinßs, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213û219 (2013).
|
| |
|
| |
| Hybridization protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinßs, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213û219 (2013).
|
| Scan protocol |
Agilent G2600D Microarray Scanner. Images were quantified using Agilent Feature Extraction Software (version 11.5.1.1).
|
| Description |
E5 strain gametocytes with disrupted ap2-g2 gene
AP2-G2::KO GC TP8
|
| Data processing |
Agilent Feature Extraction Software (v 11.5.1.1) was used for background subtraction.
|
| |
|
| Submission date |
Nov 05, 2020 |
| Last update date |
Feb 02, 2021 |
| Contact name |
Manuel Llinas |
| E-mail(s) |
manuel@psu.edu
|
| Phone |
8148673444
|
| Organization name |
Penn State University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Manuel Llinas Lab
|
| Street address |
Millennium Science Complex, Pollock Rd
|
| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL15130 |
| Series (2) |
| GSE160924 |
Plasmodium falciparum PfAP2-G2 KO gametocyte microarray timecourse |
| GSE160937 |
The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation |
|
