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Sample GSM4889918 Query DataSets for GSM4889918
Status Public on Nov 10, 2020
Title LBW female liver, biological rep4
Sample type RNA
 
Source name guinea pig liver at postnatal day 150
Organism Cavia porcellus
Characteristics strain: Dunkin Hartley
gender: Female
treatment: Uterine artery ablation-induced low birth weight (LBW)
age: postnatal day 150
tissue: Liver
Treatment protocol At postnatal day 150, offspring of each sex were fasted overnight, euthanized via CO2 inhalation in the morning (~ 10 am) of the following day and tissue collection conducted. The whole liver was removed and weighed immediately and the right lobe liver was frozen in liquid nitrogen and stored at −80 °C.
Growth protocol Uterine artery ablation-induced low birth weight (LBW) and normal birth weight (NBW) male and female guinea pig offspring were fed a control diet from weaning until postnatal day 150.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA in livers was performed using the RNeasy Plus Universal Mini Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Sense-strand cDNA was synthesized from 100 ng of total RNA, and fragmentation and labeling were performed to produce ss-cDNA with the GeneChip® WT Terminal Labeling Kit according to manufacturer’s instructions (Thermo Fisher Scientific).
 
Hybridization protocol After fragmentation and labeling, 2.1 µg cDNA was hybridized onto Guinea Pig Gene 1.1 ST Array Plate (Thermo Fisher Scientific).
Scan protocol GeneChips were scanned using the GeneTitan® Instrument (Thermo Fisher Scientific).
Description 56_TRT050A01A01_01
Gene expression data from young adult offspring fed a control diet
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) (version 4.0, Life Technologies) using Affymetrix default analysis settings and Summarization Method as normalization method.
 
Submission date Nov 09, 2020
Last update date Nov 10, 2020
Contact name Ousseynou Sarr
E-mail(s) osarr@uwo.ca
Phone 2262685936
Organization name Western universitry
Department Physiology & Pharmacology
Street address 1811 Creekside Street
City London
State/province United States
ZIP/Postal code N5X4L8
Country Canada
 
Platform ID GPL29378
Series (1)
GSE161124 Expression Data from Young Uteroplacental Insufficiency-induced Low Birth Weight Adult Guinea Pig Offspring

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
19960001 2.23763
19960003 2.43416
19960005 3.15982
19960007 1.59454
19960009 1.55338
19960011 0.7964
19960013 1.66986
19960015 2.54332
19960017 1.17672
19960019 1.26529
19960021 1.08336
19960023 0.75907
19960025 1.21807
19960027 2.69826
19960029 1.73115
19960031 2.19723
19960033 2.76298
19960035 0.87778
19960037 1.30809
19960039 1.95845

Total number of rows: 28968

Table truncated, full table size 477 Kbytes.




Supplementary file Size Download File type/resource
GSM4889918_56_TRT050A01A01_01.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM4889918_56_TRT050A01A01_01.rma-gene-full.chp.gz 186.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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