|
Status |
Public on Jun 20, 2023 |
Title |
257236329286-6 |
Sample type |
RNA |
|
|
Source name |
siControl#2 + TGF_A
|
Organism |
Homo sapiens |
Characteristics |
cell type: Normal lung fibroblasts (NHLF) amount in (ug): 0.2 rin number: 9 transfection: siControl#2 + TGF
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen - miRNeasy
|
Label |
Cy3
|
Label protocol |
Agilent - Low RNA input QuickAmp
|
|
|
Hybridization protocol |
Agilent : 200.0 ng at 65 degree_C during 17 hours
|
Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
Data processing |
Feature extraction data processing. Quantile normalization and paired differential analysis in R / Bioconductor.
|
|
|
Submission date |
Nov 12, 2020 |
Last update date |
Jun 20, 2023 |
Contact name |
Kevin Lebrigand |
Organization name |
IPMC/CNRS
|
Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
Street address |
660 route des lucioles
|
City |
Valbonne - Sophia-Antipolis |
ZIP/Postal code |
06560 |
Country |
France |
|
|
Platform ID |
GPL21185 |
Series (1) |
GSE161364 |
Impact of PRRX1 knockdown on the transcriptome of normal human lung fibroblasts (NHLF) in the presence or the absence of TGF-β |
|