|
| Status |
Public on Jun 20, 2023 |
| Title |
257236329286-8 |
| Sample type |
RNA |
| |
|
| Source name |
PRRX1 siRNA#2 + TGF_A
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Normal lung fibroblasts (NHLF)
amount in (ug): 0.2
rin number: 9
transfection: PRRX1 siRNA#2 + TGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen - miRNeasy
|
| Label |
Cy3
|
| Label protocol |
Agilent - Low RNA input QuickAmp
|
| |
|
| Hybridization protocol |
Agilent : 200.0 ng at 65 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Data processing |
Feature extraction data processing. Quantile normalization and paired differential analysis in R / Bioconductor.
|
| |
|
| Submission date |
Nov 12, 2020 |
| Last update date |
Jun 20, 2023 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE161364 |
Impact of PRRX1 knockdown on the transcriptome of normal human lung fibroblasts (NHLF) in the presence or the absence of TGF-β |
|
