|
| Status |
Public on Jan 06, 2010 |
| Title |
Case#7 |
| Sample type |
RNA |
| |
|
| Source name |
Arachnoid cyst
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
age: 44
anatomical region: left temporal fossa
galassi grade: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiuagen Rneasy minikit (Quiagen, Germany).
|
| Label |
digoxigenin (DIG)
|
| Label protocol |
Labeling was done with the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit using 2 ug high quality total RNA.
|
| |
|
| Hybridization protocol |
Fifteen ug mRNA was hybridized to the ABI Human Genome Survey Microarray v1.
|
| Scan protocol |
DIG-labeled targets hybridize to the probes on the arrays and anti-digoxigenin-alkaline phosphatase is added to bind digoxigenin in cDNA targets. The ABI 1700 Expression Array System (ABI, U.S) was used to scan the arrays for chemiluminescence.
|
| Description |
AC_2008-010
|
| Data processing |
Signal intensities generated with the ABI 1700 Expression Array System (Applied Biosystems, U.S) were imported into the the J-Express Pro v2.7 software (MolMine AS, Norway). Interarray quantile normalization was performed, and all control spots and flagged spots were removed. unsupervised cluster analysis and significance analysis of microarrays with 400 and 1000 permutations were then performed.
|
| |
|
| Submission date |
Jan 04, 2010 |
| Last update date |
Jan 06, 2010 |
| Contact name |
Mads Aarhus |
| E-mail(s) |
mads.aarhus@helse-bergen.no
|
| Phone |
99390832
|
| Fax |
99390832
|
| Organization name |
Haukeland University Hospital
|
| Department |
Center for Medical Genetics and Molecular Medicine
|
| Street address |
Jonas Lies v 65
|
| City |
Bergen |
| ZIP/Postal code |
5021 |
| Country |
Norway |
| |
|
| Platform ID |
GPL9851 |
| Series (1) |
| GSE19727 |
Molecular profiling of temporal fossa arachnoid cysts |
|
