|
| Status |
Public on Nov 26, 2020 |
| Title |
SH-SY5Y WT+FCCP_2 |
| Sample type |
protein |
| |
|
| Source name |
SH-SY5Y WT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SH-SY5Y
treatment: FCCP
mutation in sacs: WT
|
| Treatment protocol |
Cell lines were treated with CRISPR/Cas9 gene editing to produce clones harboring a loss-of function mutation in SACS; both cell lines and primary fibroblasts were treated with 20 μM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) for 2 hours to induce mitochondrial damage and fragmentation.
|
| Growth protocol |
Cells were grown at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium, containing 10% FBS, 4.5 g/L glucose and 1% antibiotics/antimycotics; primary cells from humans were grown at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium, containing 10% FBS, 4.5 g/L glucose and 1% antibiotics/antimycotics.
|
| Extracted molecule |
protein |
| Extraction protocol |
All samples (fibroblasts and cell models) were washed twice with PBS 1X and then homogenized in M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific, Rodano (MI), Italy) containing inhibitors of proteases (Roche Diagnostics, Monza (MB), Italy), following the manufacturer’s standard protocol.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed at Somalogic
|
| |
|
| Hybridization protocol |
Hybridization was performed at Somalogic
|
| Scan protocol |
Agilent scanner for microarrays
|
| Data processing |
Sample data is rst normalized to remove hybridization variation within a run followed by median normalization across all samples to remove other assay biases within the run and finally calibrated to remove assay differences between runs.
|
| |
|
| Submission date |
Nov 25, 2020 |
| Last update date |
Nov 26, 2020 |
| Contact name |
giovanna chiorino |
| E-mail(s) |
giovanna.chiorino@gmail.com
|
| Organization name |
Fondo Edo Tempia
|
| Department |
Cancer Genomics
|
| Street address |
via malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23119 |
| Series (1) |
| GSE162132 |
Functional Network Profiles In Arsacs Disclosed By Aptamer-Based Proteomic Technology |
|
