|
| Status |
Public on Oct 31, 2021 |
| Title |
Uninfected untreated rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
dTHP-1 cells uninfected untreated
|
| Organism |
Homo sapiens |
| Characteristics |
infection: uninfected
treatment: untreated
cell line: differentiated THP-1 cells
|
| Treatment protocol |
dTHP-1 cells were cultured in 6 well plated with IA, DI or without any treatment for 24 hours. Followed by 24 hour pre-treatment, cell were either infected or not with Influena A virus and kept in respective treatments for 12 hours.
|
| Growth protocol |
THP1 were cultured in RPMI for media supplemented with 10% FCS and glutamax. THP1 cell were stimulated with PMA for differentation for 48 hours.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNA was extracted and purified from dTHP-1 cells using MN NucleoSpin RNA kit.
Complementary RNA (cRNA) amplification and coversion to biotinylated double-stranded cDNA (ds-cDNA) was done by Clariom™ S Pico Assay, human Affymetrix protocol from10 ng total RNA (GeneChip® Pico UserGuide 703308 Rev. 4 Affymetrix).
|
| Label |
biotin
|
| Label protocol |
affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was done for 16 hrs at conditions recommended by the manufacturer. Clariom™ S chips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000. Image analysis was done by Affymetrix® GeneChip® Command Console® Software (AGCC) and Affymetrix® Expression Console™ Software
|
| Description |
Untreated C
Human cell line without genetic modifications
Gene expression data from dTHP-1 cells uninfected untreated for 12 hours
|
| Data processing |
Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions.
|
| |
|
| Submission date |
Nov 27, 2020 |
| Last update date |
Oct 31, 2021 |
| Contact name |
Frank Pessler |
| E-mail(s) |
frank.pessler@twincore.de
|
| Phone |
+49(511)220027167
|
| Organization name |
Center for Experimental and Clinical Infection Research GmbH
|
| Department |
Experimental Infection Research
|
| Lab |
Research Group Biomarkers for Infectious Diseases
|
| Street address |
Feodor-Lynen-Str. 7-9
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE162261 |
Expression data from differentiated THP-1 (dTHP-1) cell line infected with influenza A virus (IAV) and treated with/without Itaconate |
|
