|
| Status |
Public on Dec 07, 2023 |
| Title |
proximal femur periosteum, LCPD3 |
| Sample type |
RNA |
| |
|
| Source name |
LCPD_periosteum from proximal femur
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: patient with Legg-Calve-Perthes disease (LCPD)
gender: male
age: 8-years
tissue: periosteum from proximal femur
|
| Treatment protocol |
Periosteum specimens were biopsied from the proximal femur of each patient during surgery and transferred to liquid nitrogen immediately after being washed with saline
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the Qiagen's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16h at 48℃ on Affymetrix HTA2.0 Array.Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using the Affymatrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from proximal femur periosteum of a 8-year old boy with LCPD
|
| Data processing |
The data were analyzed with RMA (Robust Multiarray Average) using Affymetrix default analysis settings and global scaling as quantile normalization. The summarization mean target intensity of each array was arbitrarily set to 100, Pvalue≤0.05
|
| |
|
| Submission date |
Dec 07, 2020 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Xin Tang |
| Organization name |
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Street address |
1277 jiefang avenue
|
| City |
Wuhan |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE162766 |
lncRNA and mRNA expression data of periosteum from patients with Legg-Calve-Perthes disease (LCPD) and Control patients (with femoral fracture/coxa valga) |
|
