|
| Status |
Public on May 11, 2011 |
| Title |
Mac8 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Blood Whole Genome Extraction - NEPC
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: Blood
subject: 273
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Roughly 6 mL of venous blood was collected in EDTA-Vacutainer tubes during routine physicals in which blood testing was already ongoing. Genomic DNA was then isolated and purified using Flexigene kits (Qiagen, Valencia, CA) following standard manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| Channel 2 |
| Source name |
Blood Whole Genome Extraction - NEPC
|
| Organism |
Macaca mulatta |
| Characteristics |
subject: 354
tissue: Blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Roughly 6 mL of venous blood was collected in EDTA-Vacutainer tubes during routine physicals in which blood testing was already ongoing. Genomic DNA was then isolated and purified using Flexigene kits (Qiagen, Valencia, CA) following standard manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| |
| Hybridization protocol |
Test individual DNAs were labeled with Cy5 and co-hybridized with Cy3-labeled DNA from the reference individual (R354) using manufacturer's protocols.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.10.7).
|
| Description |
no additional information
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jan 13, 2010 |
| Last update date |
May 11, 2011 |
| Contact name |
Omer Gokcumen |
| E-mail(s) |
gokcumen@gmail.com
|
| Phone |
7166454937
|
| Organization name |
University at Buffalo
|
| Department |
Biological Sciences
|
| Street address |
109 Cooke Hall
|
| City |
Buffalo |
| State/province |
New York |
| ZIP/Postal code |
14260 |
| Country |
USA |
| |
|
| Platform ID |
GPL9903 |
| Series (1) |
|
