|
| Status |
Public on Dec 14, 2023 |
| Title |
adult population at day 1 of adulthood, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Caenorhabditis elegans Day 1 wt adults
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: synchronized population
genotype: wild type, N2 Bristol
age: Day 1 of adulthood
|
| Treatment protocol |
M9 buffer was applied, the nematodes were all grown following the standard procedure for wt strain and aged without any further interventions.
|
| Growth protocol |
Standard procedures for strain maintenance and culture. C. elegans strain N2 provided by the CGC. Cultures were maintained at 20⁰C in fresh nematode growth medium (NGM) plates seeded with the OP50 strain of Escherichia coli.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on C. elegans GeneChip Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from population of C. elegans at day 1 of adulthood
|
| Data processing |
The data were backgroung corrected, quantile-normalized, log2 transfrormed with oligo::rma() function using R in R-studio environment.
|
| |
|
| Submission date |
Dec 15, 2020 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Niki Chondrogianni |
| E-mail(s) |
nikichon@eie.gr
|
| Organization name |
National Hellenic Research Foundation
|
| Department |
ICB - Institute of Chemical Biology
|
| Lab |
Chondrogianni
|
| Street address |
Leof. Vasileos Konstantinou 48
|
| City |
Athens |
| State/province |
Attiki |
| ZIP/Postal code |
11635 |
| Country |
Greece |
| |
|
| Platform ID |
GPL28002 |
| Series (1) |
| GSE163253 |
Expression data from ageing wt N2 Bristol Caenorhabditis elegans population. |
|
