|
| Status |
Public on Jan 20, 2010 |
| Title |
intestine, ACD-infected, biol rep3 |
| Sample type |
RNA |
| |
|
| Source name |
infected intestine, ACD, 4 hours
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
age: 5 days
tissue: intestine
v. cholerae infection: ACD
infection time: 4 hours
|
| Treatment protocol |
5-day-old infant CD1 mice were infected with 5 x 10^9 V. cholerae for 4 hours. Intestines were harvested and homogenized in TriReagent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriReagent (Invitrogen), per manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization, Wash, Stain Kit.
|
| Scan protocol |
Gene Array Scanner 7000, Affymetrix Microarray Suite Software.
|
| Description |
ACD-3
V. cholerae strain also referred to as ATM-6, lacks background toxins rtxA hlyA hapA and the actin cross-linking domain (ACD) of vgrG-1.
|
| Data processing |
Affymetrix Expression Console, RMA.
|
| |
|
| Submission date |
Jan 19, 2010 |
| Last update date |
Jan 19, 2010 |
| Contact name |
Amy Ma |
| Organization name |
Harvard Medical School
|
| Department |
Microbiology
|
| Street address |
200 Longwood Ave D1-411
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19946 |
In vivo actin cross-linking induced by type VI secretion system is associated with intestinal inflammation |
|
