|
| Status |
Public on Apr 13, 2021 |
| Title |
KPC-Snail;Twist cKO YFP+ cancer cells from liver - mouse 407P |
| Sample type |
RNA |
| |
|
| Source name |
mouse KPC-ST YFP+ pancreatic cancer cells
|
| Organism |
Mus musculus |
| Characteristics |
eartag: 407P
gender: female
genotype: Pdx1-Cre; KrasG12D; p53R172H/+; Snai1F/F; Twist1F/F ; Rosa26-LSL-EYFPF/F; αSMA-RFP
tumor type: metastatic
|
| Treatment protocol |
tissue was minced with scissors in a 1.5 mL tube, and incubated at 37°C in 4 ug/mL Collagenase IV (Gibco, 17104019) and 4 ug/mL Dispase II (Gibco, 17105041) in serum-free RPMI in a 6well dish for 1 hour. Cells were resuspended in 10 mL of complete media (RPMI with 20% FBS and 1% PSA) and strained through a 70 um strainer and pelleted to removed digestion media. the pelleted cells were washed twice in PBS, resuspended in FACS buffer (PBS with 2% FBS) and sorted for YFP positivity by flow cytometry (BD FACSAria IIIu Cell Sorter).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted by the PicoPure RNA isolation kit (Applied Biosystems) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
whole transcriptome analysis was performed using the WT Plus Assay (Applied Biosystems) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
whole transcriptome analysis was performed using the WT Plus Assay (Applied Biosystems) according to the manufacturer's instructions.
|
| Scan protocol |
Mouse Clariom D array (Applied Biosystems)
|
| Data processing |
TAC4.0 version 2 software
|
| |
|
| Submission date |
Jan 11, 2021 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Raghu Kalluri |
| E-mail(s) |
rkalluri@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Cancer Biology
|
| Street address |
1881 East Road
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE164612 |
Expression data from stabilized epithelial pancreatic cancer cells |
|
