|
| Status |
Public on Apr 25, 2010 |
| Title |
CD4_totalRNA_RNAseq |
| Sample type |
SRA |
| |
|
| Source name |
CD4+ T cells, RNA_Seq
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD4+ T cells were purified from human blood using human CD4+ T cell isolation kit II kits (Miltenyi).
Total RNAs were prepared using miRNeasy kit from Qiagen. rRNA was depleted from total RNA by hybridization to biotinylated oligonucleotides using Invitrogen Ribominus kit. The resulting RNAs were reverse-transcribed using random primers, converted to double-stranded cDNAs, sonicated and ligated to Solexa adaptors for sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
RNA-seq experiment for total RNA in CD4+ cells
|
| Data processing |
Illumina Genome Analyzer Pipeline produced short sequence reads that were matched to the hg18 human genome using ELAND alignment algorithm.
|
| |
|
| Submission date |
Jan 27, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Iouri Chepelev |
| E-mail(s) |
ichepelev@gmail.com
|
| Organization name |
US Department of Veterans Affairs Medical Center
|
| Street address |
3200 Vine St
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45220 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (2) |
| GSE20071 |
Pol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (II) |
| GSE20072 |
Pol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes |
|
| Relations |
| SRA |
SRX017603 |
| BioSample |
SAMN00009807 |
| Named Annotation |
GSM501716_CD4_totalRNA.bed.gz |
