|
| Status |
Public on Jun 25, 2010 |
| Title |
Calcium radula rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Pooled calcium depositing radula sac tissue
|
| Organism |
Acanthopleura hirtosa |
| Characteristics |
origin: Wild
tissue: Calcium radula sac
age: Adult
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol solution was used to extract total RNA from the chiton radula sac
|
| Label |
Cy5
|
| Label protocol |
One microgram of total RNA from chiton radule sac was labeled using the Kreatech ampULSe kit to generate Cy5 labeled targets.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the standard CombiMatrix protocol for single channel 4x2k arrays described in detail at www.combimatrix.com.
|
| Scan protocol |
The Cy5- hybridized chip was then scanned using Genepix 4000B microarray scanner (Axon Instruments, CA, USA) according to manufacturer's instructions.
|
| Description |
none
|
| Data processing |
Data was pre-processed by global means normalisation.
|
| |
|
| Submission date |
Jan 29, 2010 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Luke Gardner |
| E-mail(s) |
lgardner@stanford.edu
|
| Phone |
8316556237
|
| Organization name |
Stanford University
|
| Street address |
120 Ocean View Blvd, Hopkins Marine Station
|
| City |
Pacific Grove |
| State/province |
California |
| ZIP/Postal code |
93950 |
| Country |
USA |
| |
|
| Platform ID |
GPL9992 |
| Series (1) |
| GSE20105 |
Molecular influences relating to the development and biomineralization of the chiton radula, Acanthopleura histosa |
|
