|
Status |
Public on Jun 25, 2010 |
Title |
Calcium radula rep4 |
Sample type |
RNA |
|
|
Source name |
Pooled calcium depositing radula sac tissue
|
Organism |
Liolophura hirtosa |
Characteristics |
origin: Wild tissue: Calcium radula sac age: Adult
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol solution was used to extract total RNA from the chiton radula sac
|
Label |
Cy5
|
Label protocol |
One microgram of total RNA from chiton radule sac was labeled using the Kreatech ampULSe kit to generate Cy5 labeled targets.
|
|
|
Hybridization protocol |
Hybridization was performed according to the standard CombiMatrix protocol for single channel 4x2k arrays described in detail at www.combimatrix.com.
|
Scan protocol |
The Cy5- hybridized chip was then scanned using Genepix 4000B microarray scanner (Axon Instruments, CA, USA) according to manufacturer's instructions.
|
Description |
none
|
Data processing |
Data was pre-processed by global means normalisation.
|
|
|
Submission date |
Jan 29, 2010 |
Last update date |
Jan 29, 2010 |
Contact name |
Luke Gardner |
E-mail(s) |
lgardner@stanford.edu
|
Phone |
8316556237
|
Organization name |
Stanford University
|
Street address |
120 Ocean View Blvd, Hopkins Marine Station
|
City |
Pacific Grove |
State/province |
California |
ZIP/Postal code |
93950 |
Country |
USA |
|
|
Platform ID |
GPL9992 |
Series (1) |
GSE20105 |
Molecular influences relating to the development and biomineralization of the chiton radula, Acanthopleura histosa |
|