|
| Status |
Public on Jan 02, 2022 |
| Title |
Copper Low 1 |
| Sample type |
RNA |
| |
|
| Source name |
Daphnia magna gut
|
| Organism |
Daphnia magna |
| Characteristics |
tissue: Dissected guts
|
| Treatment protocol |
water exposures according to the manuscript linked
|
| Growth protocol |
daphnias were cultured and grown according to Barata and Baird, 200
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to Soetaert et al. 2007
|
| Label |
Cy3
|
| Label protocol |
according to manufacturer protocols Low input Quick Amp lebelling
|
| |
|
| Hybridization protocol |
according to manufacturer protocols Low input Quick Amp lebelling
|
| Scan protocol |
according to manufacturer protocols Low input Quick Amp lebelling
|
| Description |
Copper H2 1
Agilent-079797 Daphnia magna DGC v2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.5 (Agilent) using default parameters (protocol GE1_105_Dec08 and Grid:079797_D_F_20151120)to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jan 28, 2021 |
| Last update date |
Jan 02, 2022 |
| Contact name |
Bruno Campos |
| E-mail(s) |
brunocampus@gmail.com
|
| Organization name |
IDAEA-CSIC
|
| Department |
Environmental Chemistry
|
| Street address |
jordi girona, 18, 18
|
| City |
Barcelona |
| ZIP/Postal code |
08034 |
| Country |
Spain |
| |
|
| Platform ID |
GPL29663 |
| Series (1) |
| GSE165726 |
Transcriptomic respponses in the gut in Daphnia magna across toxic chemicals that inhibit feeding and food limiting conditions |
|
