|
| Status |
Public on Feb 02, 2011 |
| Title |
5 Ko |
| Sample type |
RNA |
| |
|
| Source name |
LSK cell from Gfi1 knockout mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
|
| Treatment protocol |
LSKs were sorted by FACS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Rneasy mini kit (QIAGEN)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Arrays were scanned in a GeneArray Scanner (Agilent).
|
| Description |
Gene expression data from LSK cell from Gfi1 knockout mice
|
| Data processing |
The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 11, 2010 |
| Last update date |
Feb 02, 2011 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE20282 |
Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases |
|
