|
| Status |
Public on Mar 01, 2021 |
| Title |
JRJ024: TP53 mutant thymocytes non-irradiated, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
thymocytes from non-irradiated mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J TP53R337H/R337H
tissue: thymocytes from non-irradiated mouse
|
| Treatment protocol |
Thymocytes were obtained from WT or p53 mutant mice 6h after treatment with 5 Gy whole body radiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy mini columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Sense target cDNA was prepared from 100ng of total RNA using the Ambion® WT Expression Kit (Life Technologies P/N 4411973). cDNA was fragmented and biotin-labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix P/N 900671).
|
| |
|
| Hybridization protocol |
Five micrograms of biotinylated cDNA was hybridized for 16h at 45C on a Mouse Gene 2.0 ST GeneChip array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.6
|
| |
|
| Submission date |
Feb 16, 2021 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE166819 |
The common germline TP53-R337H mutation is hypomorphic and confers incomplete tumor penetrance and late tumor onset in a mouse model |
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