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Status |
Public on Mar 01, 2021 |
Title |
JRJ030: TP53 mutant thymocytes irradiated, replicate 2 |
Sample type |
RNA |
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Source name |
thymocytes from irradiated mouse
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Organism |
Mus musculus |
Characteristics |
strain: C57BL6/J TP53R337H/R337H tissue: thymocytes from irradiated mouse
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Treatment protocol |
Thymocytes were obtained from WT or p53 mutant mice 6h after treatment with 5 Gy whole body radiation
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Rneasy mini columns (Qiagen)
|
Label |
biotin
|
Label protocol |
Sense target cDNA was prepared from 100ng of total RNA using the Ambion® WT Expression Kit (Life Technologies P/N 4411973). cDNA was fragmented and biotin-labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix P/N 900671).
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Hybridization protocol |
Five micrograms of biotinylated cDNA was hybridized for 16h at 45C on a Mouse Gene 2.0 ST GeneChip array.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
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Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.6
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Submission date |
Feb 16, 2021 |
Last update date |
Mar 01, 2021 |
Contact name |
Geoffrey Neale |
E-mail(s) |
geoffrey.neale@stjude.org
|
Organization name |
St Jude Childrens Research Hospital
|
Department |
Hartwell Center
|
Street address |
262 Danny Thomas Place
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
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Platform ID |
GPL16570 |
Series (1) |
GSE166819 |
The common germline TP53-R337H mutation is hypomorphic and confers incomplete tumor penetrance and late tumor onset in a mouse model |
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