|
| Status |
Public on Oct 19, 2010 |
| Title |
Control-264hrs (251506410303_1_3) |
| Sample type |
RNA |
| |
|
| Source name |
Spinal cord, 5mm caudal from lesion site
|
| Organism |
Danio rerio |
| Characteristics |
tissue: spinal cord
treatment: sham injury (control)
time: 264hrs
age: 6 months
|
| Treatment protocol |
Spinal cord transection performed on anesthetized (0.033% aminobenzoic acid ethymethylester, MS222) between two vertebraa at a level halfway between dorsal fin and operculum, about 4 mm caudal to brainstem/spinal cord transitional zone. Wounds were sealed with Histacryl. Control treatment (sham injury) was performed by making the incision without spinal cord lesion.
|
| Growth protocol |
Before surgery fish were kept in groups of 10 under conditions of 14 hrs light/10 hrs dark, after surgery, kept individually
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spinal cord segments, dissected 5mm caudal from injury site, were pooled from 5 animals and prepared using Qiagen RNeasy Micro Kit.
|
| Label |
Cy3
|
| Label protocol |
NuGEN WT-Ovation kit
|
| |
|
| Hybridization protocol |
Standard Agilent protocol
|
| Scan protocol |
Standard Agilent protocol
|
| Description |
spinal cord, 5mm distal to lesion site, pooled from 5 animals
|
| Data processing |
Agilent feature extraction (version 9.5.3.1 3) followed by importing into GeneSpring GX 7
|
| |
|
| Submission date |
Feb 22, 2010 |
| Last update date |
Oct 19, 2010 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL7302 |
| Series (1) |
|
