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Sample GSM513253 Query DataSets for GSM513253
Status Public on Feb 25, 2010
Title 0 Dis VIII batch RNA
Sample type RNA
 
Channel 1
Source name wt
Organism Saccharomyces cerevisiae
Characteristics strain name: wt
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy3
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
Channel 2
Source name Dis VIII
Organism Saccharomyces cerevisiae
Characteristics strain name: Dis VIII
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy5
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
 
Hybridization protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
Scan protocol Pixel Size : 5
Scan Date : 2009-06-19
Scan Time : 09:27:51
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description Detailed protocols are available in 'GSE20464_protocols.zip', which is linked to the Series GSE20464 record as a supplementary file.
0 Dis VIII batch RNA
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.1.3.1
Datafile type : Agilent result file
Normalizaton: Default settings of Agilent Feature Extractor
 
Submission date Feb 22, 2010
Last update date Feb 24, 2010
Contact name Maitreya J. Dunham
E-mail(s) maitreya@uw.edu
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
 
Platform ID GPL10045
Series (1)
GSE20464 Identification of Aneuploidy-Tolerating Mutations

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 .41 189.536 142.6852 458.2105 318.3684
2 -1.125 2.701292 5.892492 95.14706 97.11765
3 2.079 5.351685 1.26675 100.697 85.48485
4 .039 3617.803 3520.65 7642.512 6089.122
5 .082 3290.876 3108.743 7094.795 5461.462
6 -.248 8532.728 10134.26 18570.34 17819.37
7 .124 4979.381 4570.525 11046.33 8169.308
8 .18 9752.572 8609.875 21848.71 15451.68
10 .011 510.6757 506.9558 1255.821 1002.103
11 .773 24.4197 14.29489 146.2308 110.4103
12 -.208 2305.954 2663.601 5445.171 4944.951
13 .314 1260.006 1013.271 3035.128 1937.769
14 .223 20.35985 17.44819 138.1316 115.3421
15 -.236 283.3542 333.6856 759.1 694.7
16 .103 7974.384 7426.144 18965.97 13693.42
17 -.195 2380.554 2725.414 5737.105 5071.158
18 -.033 149.8275 153.259 446.4865 361.7838
19 .244 5759.68 4864.997 13773.76 8943.895
20 -.166 20.54983 23.05875 139.6571 122.9714
21 -.268 1077.378 1297.48 2646.415 2426.22

Total number of rows: 13048

Table truncated, full table size 585 Kbytes.




Supplementary file Size Download File type/resource
GSM513253.txt.gz 4.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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