|
| Status |
Public on Feb 25, 2010 |
| Title |
AA Evolved Disome 8.1 c1 P-lim Chemostat RNA 4x44k |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
F1 12/6/05 RNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain name: wt
|
| Biomaterial provider |
Cheryl Christianson, Dunham Lab, Princeton University
|
| Growth protocol |
Media : phosphate-limited chemostat media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
|
| Label |
Cy3
|
| Label protocol |
reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
|
| |
|
| Channel 2 |
| Source name |
F7 4/10/07 RNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain name: Evolved Disome VIII 14.1
|
| Biomaterial provider |
Cheryl Christianson, Dunham Lab, Princeton University
|
| Growth protocol |
Media : phosphate-limited chemostat media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
|
| Label |
Cy5
|
| Label protocol |
reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
|
| |
|
| |
| Hybridization protocol |
reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
|
| Scan protocol |
Pixel Size : 5
Scan Date : 2007-05-25
Scan Time : 11:19:10
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
|
| Description |
Detailed protocols are available in 'GSE20464_protocols.zip', which is linked to the Series GSE20464 record as a supplementary file.
AA Evolved Disome 8.1 c1 P-lim Chemostat RNA 4x44k
|
| Data processing |
Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.5.1.1
Datafile type : Agilent result file
Normalizaton: Default settings of Agilent Feature Extractor
|
| |
|
| Submission date |
Feb 22, 2010 |
| Last update date |
Feb 24, 2010 |
| Contact name |
Maitreya J. Dunham |
| E-mail(s) |
maitreya@uw.edu
|
| Phone |
206-543-2338
|
| Organization name |
University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Dunham Lab
|
| Street address |
Foege Building, S403B, Box 355065
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-5065 |
| Country |
USA |
| |
|
| Platform ID |
GPL7542 |
| Series (1) |
| GSE20464 |
Identification of Aneuploidy-Tolerating Mutations |
|
