|
| Status |
Public on May 18, 2021 |
| Title |
802 2020 |
| Sample type |
RNA |
| |
|
| Source name |
mammary epithelium
|
| Organism |
Mus musculus |
| Characteristics |
cell type: basal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA amounts of 25 or 50 ng, as used initially and in the 10,000 cell analysis, library preparation and amplification for 17 cycles were performed following the distributor's (Sigma-Aldrich) recommendations.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cDNA was hybridized to the Clariom S Mouse Array (901570, Thermo Scientific).
|
| Scan protocol |
The arrays were scanned on a GeneAtlas Imaging station (Affymetrix).
|
| Data processing |
After RMA normalisation and batch correction using the ComBat function of the SVA package in R, differentially expressed genes were determined using the limma package (version 3.32.10) in R and a FDR < 0.05.
|
| |
|
| Submission date |
Mar 10, 2021 |
| Last update date |
May 19, 2021 |
| Contact name |
Laudanna Carmelo |
| E-mail(s) |
carmelo.laudanna@irbbarcelona.org
|
| Organization name |
Institute for Research in Biomedicine (IRB-Barcelona)
|
| Department |
Aging and Metabolism
|
| Lab |
Stem cells and Cancer Lab
|
| Street address |
Baldiri i Reixac, 10
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE149978 |
Repression of endogenous retroviruses is required for mammary gland development and homeostasis |
| GSE168624 |
Repression of endogenous retroviruses is required for mammary gland development and homeostasis [microarray] |
|
