|
| Status |
Public on Apr 15, 2010 |
| Title |
Calu3, replicate 8 |
| Sample type |
RNA |
| |
|
| Source name |
Calu3, air-liquid interface
|
| Organism |
Homo sapiens |
| Characteristics |
condition: In vitro
surface: Air-liquid interface
localization: Other
sample date: Other
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix HsAirway array. The arrays were washed and stained using the Affymetrix Model 450 Fluidics Station.
|
| Scan protocol |
The arrays were scanned using the Affymetrix Model 3000 scanner
|
| Description |
Gene expression data from airway epithelia
|
| Data processing |
Raw data .cel files were imported into Partek Genomics Suite v 6.4 and normalized using the RMA algorithm.
|
| |
|
| Submission date |
Feb 23, 2010 |
| Last update date |
Feb 23, 2010 |
| Contact name |
Bartley Joseph Brown |
| E-mail(s) |
bbrown@eng.uiowa.edu
|
| Phone |
319 335 6432
|
| Organization name |
The University of Iowa
|
| Lab |
CBCB
|
| Street address |
5317
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL10097 |
| Series (1) |
| GSE20502 |
Human airway epithelial cell comparisons |
|
