NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM515207 Query DataSets for GSM515207
Status Public on Apr 15, 2010
Title In vivo human airway epithelia from brush biopsy, biological replicate 12
Sample type RNA
 
Source name Human airway epithelia in vivo from brush biopsy, trachea
Organism Homo sapiens
Characteristics condition: In vivo
surface: Air-liquid interface
localization: Trachea
sample date: 9/1/06
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix HsAirway array. The arrays were washed and stained using the Affymetrix Model 450 Fluidics Station.
Scan protocol The arrays were scanned using the Affymetrix Model 3000 scanner
Description Gene expression data from airway epithelia
Data processing Raw data .cel files were imported into Partek Genomics Suite v 6.4 and normalized using the RMA algorithm.
 
Submission date Feb 23, 2010
Last update date Feb 23, 2010
Contact name Bartley Joseph Brown
E-mail(s) bbrown@eng.uiowa.edu
Phone 319 335 6432
Organization name The University of Iowa
Lab CBCB
Street address 5317
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL10097
Series (1)
GSE20502 Human airway epithelial cell comparisons

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
1294_at 7.86965
1552257_a_at 7.63505
1552258_at 4.76019
1552261_at 7.80312
1552266_at 4.6958
1552269_at 8.28781
1552272_a_at 5.80767
1552281_at 6.65664
1552283_s_at 8.40723
1552286_at 5.68558
1552291_at 6.01818
1552296_at 6.94098
1552299_at 6.88696
1552301_a_at 4.74034
1552310_at 7.83264
1552312_a_at 5.74968
1552318_at 3.75273
1552326_a_at 10.5279
1552327_at 4.11287
1552330_at 6.75752

Total number of rows: 23064

Table truncated, full table size 426 Kbytes.




Supplementary file Size Download File type/resource
GSM515207.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap