|
Status |
Public on Apr 15, 2010 |
Title |
In vivo human airway epithelia from brush biopsy, biological replicate 12 |
Sample type |
RNA |
|
|
Source name |
Human airway epithelia in vivo from brush biopsy, trachea
|
Organism |
Homo sapiens |
Characteristics |
condition: In vivo surface: Air-liquid interface localization: Trachea sample date: 9/1/06
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen)
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
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|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix HsAirway array. The arrays were washed and stained using the Affymetrix Model 450 Fluidics Station.
|
Scan protocol |
The arrays were scanned using the Affymetrix Model 3000 scanner
|
Description |
Gene expression data from airway epithelia
|
Data processing |
Raw data .cel files were imported into Partek Genomics Suite v 6.4 and normalized using the RMA algorithm.
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|
|
Submission date |
Feb 23, 2010 |
Last update date |
Feb 23, 2010 |
Contact name |
Bartley Joseph Brown |
E-mail(s) |
bbrown@eng.uiowa.edu
|
Phone |
319 335 6432
|
Organization name |
The University of Iowa
|
Lab |
CBCB
|
Street address |
5317
|
City |
Iowa City |
State/province |
IA |
ZIP/Postal code |
52242 |
Country |
USA |
|
|
Platform ID |
GPL10097 |
Series (1) |
GSE20502 |
Human airway epithelial cell comparisons |
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