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Sample GSM515217 Query DataSets for GSM515217
Status Public on Apr 15, 2010
Title Primary culture of human airway epithelia submerged in growth media, biological replicate 6
Sample type RNA
 
Source name Primary culture of human airway epithelia, submerged in nutrient media
Organism Homo sapiens
Characteristics condition: In vitro
surface: Submerged
localization: Other
sample date: 6/8/04
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix HsAirway array. The arrays were washed and stained using the Affymetrix Model 450 Fluidics Station.
Scan protocol The arrays were scanned using the Affymetrix Model 3000 scanner
Description Gene expression data from airway epithelia
Data processing Raw data .cel files were imported into Partek Genomics Suite v 6.4 and normalized using the RMA algorithm.
 
Submission date Feb 23, 2010
Last update date Feb 23, 2010
Contact name Bartley Joseph Brown
E-mail(s) bbrown@eng.uiowa.edu
Phone 319 335 6432
Organization name The University of Iowa
Lab CBCB
Street address 5317
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL10097
Series (1)
GSE20502 Human airway epithelial cell comparisons

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
1294_at 6.7528
1552257_a_at 8.86452
1552258_at 5.17889
1552261_at 5.42643
1552266_at 4.54824
1552269_at 4.01732
1552272_a_at 5.41495
1552281_at 7.44849
1552283_s_at 6.37564
1552286_at 7.1208
1552291_at 7.57779
1552296_at 4.09465
1552299_at 4.30657
1552301_a_at 5.20433
1552310_at 7.68566
1552312_a_at 5.57988
1552318_at 3.48666
1552326_a_at 5.79394
1552327_at 4.15411
1552330_at 6.14139

Total number of rows: 23064

Table truncated, full table size 426 Kbytes.




Supplementary file Size Download File type/resource
GSM515217.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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