|
| Status |
Public on Mar 13, 2021 |
| Title |
PBS-1_1dose |
| Sample type |
SRA |
| |
|
| Source name |
Tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: B16F10 tumors
agent: PBS
dose_number: 1
|
| Treatment protocol |
PBS, unloaded EVs, CDN2 (20 µg), and exoCDN2 (0.1 µg) were injected intratumorally into B16F10 tumors and tumors were collected 24 h after dose. Additional tumors were injected again 3 days after first dose and collected 24 h after second dose.
|
| Growth protocol |
To establish syngeneic tumor models, B16F10 (1×106 cells to C57BL/6) were injected subcutaneously to the right flank of mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumors were lysed Trizol solution with mechanical disruption. RNAs were isolated by using RNeasy Lipid Tissue Mini Kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RNA-seq FASTQ files were processed using the customized bulk RNA-seq data analysis pipeline in OmicSoft ArraySuite, version 9.
Specifically, raw reads were first filtered based on quality control (QC) and then aligned to the reference genome (mouse genome mm10) using OSA.
After alignment, gene expression levels (raw read counts) were quantified by the RSEM algorithm with the mouse gene model Ensembl.R88.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw read counts for each Sample ...
|
| |
|
| Submission date |
Mar 12, 2021 |
| Last update date |
Mar 13, 2021 |
| Contact name |
Kelvin Zhang |
| Organization name |
Codiak Biosciences
|
| Street address |
35 CambridgePark Drive, Suite 500
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02140 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE168784 |
ExoSTING, an extracellular vesicle loaded with STING agonists, promotes tumor immune surveillance |
|
| Relations |
| BioSample |
SAMN18277108 |
| SRA |
SRX10329672 |
