|
| Status |
Public on Mar 09, 2010 |
| Title |
2nd_generation_son |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: test
generation: 2nd
gender: male
disease state (mesomelic dysplasia kantaputra): unaffected
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was prepared using the QIAamp DNA blood Midi Kit (Qiagen, Valencia, CA, US)
|
| Label |
Cy5
|
| Label protocol |
Samples (1.5 μg of DNA) were labeled with the Agilent labelling kit according to the manufacturer’s instructions.
|
| |
|
| Channel 2 |
| Source name |
Promega, Human Genomic DNA, Male (G1471)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
gender: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was prepared using the QIAamp DNA blood Midi Kit (Qiagen, Valencia, CA, US)
|
| Label |
Cy3
|
| Label protocol |
Samples (1.5 μg of DNA) were labeled with the Agilent labelling kit according to the manufacturer’s instructions.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed with the Agilent Oligo aCGH hybridiaztion kit according to the manufacturer’s instructions.
|
| Scan protocol |
Slides were scanned on an Agilent Microarray Scanner and captured images were analysed with Feature Extraction Software v 10.5.
|
| Description |
II_2
|
| Data processing |
Feature Extraction Software v 10.5 was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Mar 09, 2010 |
| Contact name |
Woong Yang Park |
| E-mail(s) |
wypark@snu.ac.kr
|
| Phone |
+82-02-740-8241
|
| Fax |
+82-02-744-4534
|
| Organization name |
Seoul National University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Molecular and Genomic Medicine
|
| Street address |
28 Yongondong, Chongnogu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10150 |
| Series (1) |
| GSE20691 |
A Novel Dominant Mesomelic Dysplasia Associated with A 1.0-Mb Microduplication of HOXD Gene Cluster at 2q31.1 |
|
