|
| Status |
Public on Apr 01, 2012 |
| Title |
6-13-64 T-cell hybridoma ICOS(-) vs ICOS(+) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
6-13-64-ICOS(-)
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 6-13-64
cell type: T-cell hybridoma
background: AKR/J x BALB/c hybrid
cell subpopulation: ICOS(-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Total RNA was reverse transcribed and labeled using SuperScript Indirect cDNA Labeling System (Invitrogen) following manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
6-13-64-ICOS(+)
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 6-13-64
cell type: T-cell hybridoma
background: AKR/J x BALB/c hybrid
cell subpopulation: ICOS(+)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was reverse transcribed and labeled using SuperScript Indirect cDNA Labeling System (Invitrogen) following manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Scanned on Scanarray Express (Perkin Elmer).
Images were quantified using QuantArray 3.0 software (Perkin Elmer).
|
| Description |
6-13-64-ICOS(-) vs. ICOS(+)
|
| Data processing |
Data processing was perfomed using GeneSpring 6.2.1 software (Silicon Genetics).
|
| |
|
| Submission date |
Mar 16, 2010 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Ryo Abe |
| Organization name |
Tokyo University of Science
|
| Department |
Research Institute for Biological Sciences
|
| Lab |
Division of Immunobiology
|
| Street address |
2669 Yamazaki
|
| City |
Noda |
| State/province |
Chiba |
| ZIP/Postal code |
278-0022 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10190 |
| Series (1) |
| GSE20922 |
Gene expression comparison between the murine T-cell hybridoma 6-13-64 ICOS(-) population and ICOS(+) population |
|
