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Status
Public on Jun 06, 2006
Title
114b-D5:D3
Sample type
RNA
Channel 1
Source name
AEC II on day 5
Organism
Rattus norvegicus
Extracted molecule
total RNA
Channel 2
Source name
AEC II on day 3
Organism
Rattus norvegicus
Extracted molecule
total RNA
Description
This is a time course study of gene expression profile in type II alveolar epithelial cells isolated from normal, hyperoxia exposed (day 0) and recovering rats (day 1, 3, 5 and 7) using parallel hybridization of DNA micoarray.
Submission date
May 24, 2005
Last update date
Jun 06, 2005
Contact name
Zhongming Chen
E-mail(s)
zhongmi@okstate.edu
Phone
405-744-0329
URL
http://www.cvm.okstate.edu/research/Facilities/LungBiologyLab/
Organization name
Oklahoma State University
Department
Deptment of Physiological Sciences
Lab
Lin Liu Lab
Street address
264 McElroy Hall
City
Stillwater
State/province
OK
ZIP/Postal code
74078
Country
USA
Platform ID
GPL1699
Series (1)
GSE2707
Gene expression profiles in AEC II during hyperoxia exposure and recovery
Data table header descriptions
ID_REF
VALUE
LOWESS normalized log2 ratio of green channel (CH1, Cy3) vs red channel (CH2, Alexa647)
CH1_MEAN
Scaled green fluorescence intensity (CH1, Cy3), percentile 5 converted to 0, percentile 99 to 1000, the rest linearly to 0~10000
CH2_MEAN
Scaled red fluorescence intensity (CH2, Alexa647), percentile 5 converted to 0, percentile 99 to 1000, the rest linearly to 0~10000
X
Y
Dia.
F3 Mean
F5 Mean
F3 Mean - B3
F5 Mean - B5
B3 Mean
B5 Mean
F3 Median
F3 Median - B3
F5 Median
F5 Median - B5
B3 Median
B5 Median
F3 SD
F5 SD
B3 SD
B5 SD
Flags
B Pixels
F Pixels
F3 % Sat.
% > B3+1SD
% > B3+2SD
% > B5+1SD
% > B5+2SD
F5 % Sat.
Index
Log Ratio (5/3)
Mean of Ratios (5/3)
Median of Ratios (5/3)
Normalize
Ratio of Means (5/3)
Ratio of Medians (5/3)
Ratios SD (5/3)
Rgn Ratio (5/3)
Rgn R? (5/3)
Sum of Means
Sum of Medians
Data table
ID_REF
VALUE
CH1_MEAN
CH2_MEAN
X
Y
Dia.
F3 Mean
F5 Mean
F3 Mean - B3
F5 Mean - B5
B3 Mean
B5 Mean
F3 Median
F3 Median - B3
F5 Median
F5 Median - B5
B3 Median
B5 Median
F3 SD
F5 SD
B3 SD
B5 SD
Flags
B Pixels
F Pixels
F3 % Sat.
% > B3+1SD
% > B3+2SD
% > B5+1SD
% > B5+2SD
F5 % Sat.
Index
Log Ratio (5/3)
Mean of Ratios (5/3)
Median of Ratios (5/3)
Normalize
Ratio of Means (5/3)
Ratio of Medians (5/3)
Ratios SD (5/3)
Rgn Ratio (5/3)
Rgn R? (5/3)
Sum of Means
Sum of Medians
AF007818
-0.61
9.256733055
37.36322391
830
23340
100
166
224
23
35
185
248
142
-1
172
-17
143
189
92
133
126
206
-50
448
80
0
15
2
12
1
0
10001
4.087
1.439
1.332
0
1.522
17
3.198
5.27
0.019
58
-18
AF027331
-0.13
21.19304673
51.17819746
1010
23340
100
220
272
72
79
182
254
151
3
231
38
148
193
164
159
113
247
-50
426
80
0
31
15
16
1
0
10002
3.663
1.243
1.414
0
1.097
12.667
4.433
7.061
0.01
151
41
AF032925
-0.81
13.39790311
48.35240742
1190
23340
100
202
306
40
70
206
320
160
-2
265
29
162
236
119
190
156
386
-50
430
80
0
13
5
8
0
0
10003
1.457
1.041
0
1.75
-14.5
4.251
4.192
0.408
110
27
AF046886
-1.09
21.19304673
74.41247115
1370
23340
100
240
376
72
153
208
297
183
15
268
45
168
223
267
449
156
332
-50
400
80
0
15
6
15
3
0
10004
1.585
1.356
1.155
0
2.125
3
4.705
1.882
0.35
225
60
S79292
-0.95
21.92384145
71.90065778
1550
23340
100
225
358
75
145
189
272
185
35
284
71
150
213
149
253
135
236
-50
426
80
0
21
11
25
7
0
10005
1.02
1.241
1.227
0
1.933
2.029
3.04
6.783
0.036
220
106
NM_053986
2.35
14.85949254
29.19983045
1730
23340
100
215
266
46
9
226
394
164
-5
229
-28
169
257
155
154
220
560
-50
419
80
0
11
3
2
0
0
10006
2.485
1.36
1.404
0
0.196
5.6
3.646
2.229
0.276
55
-33
NM_019360
-0.7
78.68223097
183.3623762
1920
23330
140
495
781
308
500
253
457
447
260
763
482
187
281
244
338
248
1072
0
550
156
0
51
16
7
0
0
10007
0.891
1.851
1.9
0
1.623
1.854
3.296
6.947
0.24
808
742
M35602
0.03
17.05187668
43.32878067
2090
23340
100
229
302
55
54
231
431
193
19
276
28
174
248
135
160
209
1239
-50
381
80
0
13
2
0
0
0
10008
0.559
1.428
1.284
0
0.982
1.474
4.762
5.975
0.233
109
47
M64391
-0.2
23.62902911
55.88784753
2270
23340
100
262
362
82
94
243
431
188
8
341
73
180
268
181
202
238
760
-50
426
80
0
18
3
0
0
0
10009
3.19
1.017
0.739
0
1.146
9.125
3.638
4.61
0.156
176
81
S48142
0.49
8.769536579
31.08369048
2450
23340
100
195
279
21
15
252
456
157
-17
231
-33
174
264
125
141
256
793
-50
426
80
0
6
0
0
0
0
10010
0.957
1.466
1.227
0
0.714
1.941
3.526
5.471
0.313
36
-50
AB050745
-0.3
10.96192072
37.99117726
2630
23340
100
235
372
30
37
282
621
187
-18
310
-25
205
335
171
266
252
1143
-50
436
80
0
10
3
1
0
0
10011
0.474
1.452
1.8
0
1.233
1.389
3.635
7.772
0.401
67
-43
NM_030838
-3.58
27.77019917
398.7503728
2820
23330
100
291
1515
99
1186
297
683
241
49
1480
1151
192
329
202
555
474
1868
0
414
80
0
8
0
10
0
0
10012
4.554
8.461
8.447
0
11.98
23.49
2.557
4.383
0.856
1285
1200
NM_017026
0.95
25.82141326
41.13094397
2990
23340
100
334
455
91
47
343
774
328
85
394
-14
243
408
190
333
385
1218
-50
414
80
0
5
1
2
0
0
10013
1.419
1.719
0
0.516
-0.165
3.657
3.986
0.493
138
71
NM_053295
-0.96
33.37295865
100.7865116
3170
23340
100
368
658
122
237
359
798
314
68
616
195
246
421
225
389
412
1241
-50
426
80
0
11
1
2
0
0
10014
1.52
2.214
1.886
0
1.943
2.868
3.305
4.428
0.4
359
263
NM_023101
-0.1
10.71832249
36.10731723
3350
23340
100
220
350
29
31
284
616
198
7
292
-27
191
319
122
221
354
1168
-50
426
80
0
0
0
0
0
0
10015
1.781
1.524
0
1.069
-3.857
4.161
4.811
0.548
60
-20
U60562
-1.29
24.11622559
90.73925807
3530
23340
100
270
475
84
205
313
611
217
31
415
145
186
270
209
335
663
1949
-50
445
80
0
1
1
1
0
0
10016
2.226
1.562
1.49
0
2.44
4.677
3.818
4.903
0.228
289
176
NM_012873
-1.36
104.0164478
358.5613589
3730
23350
100
623
1358
412
1058
306
606
477
266
1104
804
211
300
481
873
441
1334
-100
361
80
0
37
17
25
6
0
10017
1.596
2.438
2.098
0
2.568
3.023
2.469
4.278
0.404
1470
1070
M25823
0.16
19.7314573
44.89866403
3890
23340
100
270
366
66
59
322
816
237
33
312
5
204
307
175
263
529
2186
-50
398
80
0
2
0
0
0
0
10018
-2.722
1.415
1.353
0
0.894
0.152
5.078
8.834
0.562
125
38
NM_019323
0.49
26.30860974
47.09650073
4070
23340
100
278
344
93
66
273
583
250
65
302
24
185
278
177
247
327
1660
-50
426
80
0
10
1
0
0
0
10019
-1.437
0.988
1.161
0
0.71
0.369
4.038
6.517
0.564
159
89
NM_053655
4.81
10.47472425
24.80415705
4250
23340
100
231
318
28
-5
304
567
194
-9
274
-49
203
323
145
172
385
967
-50
426
80
0
3
0
0
0
0
10020
2.445
1.446
1.449
0
-0.179
5.444
3.343
3.455
0.631
23
-58
Total number of rows: 10000 Table truncated, full table size 2007 Kbytes .Supplementary data files not provided