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| Status |
Public on May 19, 2021 |
| Title |
Sample_794-AA-2 |
| Sample type |
SRA |
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| Source name |
Ventromedial and dorsomedial hypothalamus
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| Organism |
Macaca mulatta |
| Characteristics |
tissue: Ventromedial and dorsomedial hypothalamus
age: 6.1
Sex: female
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Whole Rhesus macaque brains were obtained from the Tissue Distribution Program at ONPRC. Brains were centered within a chilled brain matrix (ASI Instruments, catalog # MBM-2000C) and 2mm sliceswere obtained from rostral to caudal. Slices containing the hypothalamus were placed in saline and the PVH, ARC, VMH and DMH were punched out and samples were placed in pre-chilled tubes on dry-ice. Samples were stored at -80C until shipment on dry ice. Nuclei were isolated from frozen macaque tissue and subjected to 10x snRNA-seq.
10x 3’ Gene Expression
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
alignment, mapping, and demultiplexing with cellranger
Genome_build: Mmul_10
Supplementary_files_format_and_content: sparse matrix of counts with associated barcodes and features
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| Submission date |
Apr 16, 2021 |
| Last update date |
May 19, 2021 |
| Contact name |
Martin G Myers Jr |
| E-mail(s) |
mgmyers@umich.edu
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| Organization name |
University of Michigan
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| Street address |
1000 Wall St
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48105 |
| Country |
USA |
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| Platform ID |
GPL27943 |
| Series (2) |
| GSE172203 |
Single nucleus RNA-seq atlas of macaque ventromedial and dorsomedial hypothalamus |
| GSE172207 |
Cross-Species Analysis Defines the Conservation of Anatomically-Segregated VMH Neuron Populations |
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| Relations |
| BioSample |
SAMN18754338 |
| SRA |
SRX10614804 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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