|
| Status |
Public on May 10, 2021 |
| Title |
10X_library_WT_12hpf1 |
| Sample type |
SRA |
| |
|
| Source name |
Whole embryo_dissociated
|
| Organism |
Ciona robusta |
| Characteristics |
tissue: Whole embryo
developmental stage: 12hpf
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Embryonic time points are indicated at hours post-fertilization (hpf) for embryo development in artificial sea water at 18 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10X Chromium Single Cell 3' RNA-Seq
single-cell library was prepared following Chromium Single Cell 3' v2 Reagent kit following the manufacturer's manual.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
The feature-barcode matrices were generated using 10X Genomic Cell ranger analysis pipelines.
Genome_build: Genome Sequence, Joined-scaffold (KH), http://ghost.zool.kyoto-u.ac.jp/datas/JoinedScaffold.zip; KH gene models (ver.2013),http://ghost.zool.kyoto-u.ac.jp/datas/KH.KHGene.2013.gff3.zip
Supplementary_files_format_and_content: feature-barcode matrices, non-normalized UMI counts.
|
| |
|
| Submission date |
May 09, 2021 |
| Last update date |
May 10, 2021 |
| Contact name |
wei wang |
| E-mail(s) |
ww39@nyu.edu
|
| Phone |
+1 2129988381
|
| Organization name |
New York University
|
| Department |
Biology
|
| Street address |
100 washington square east
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
| |
|
| Platform ID |
GPL23563 |
| Series (1) |
| GSE174127 |
10X Genomics Single Cell RNAseq of wild-type Ciona robusta embryos at 12 hours post fertilization (hpf) |
|
| Relations |
| BioSample |
SAMN19081973 |
| SRA |
SRX10824621 |
