|
| Status |
Public on Sep 15, 2010 |
| Title |
pif1345_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
5 days-old seedlings, replicate 3
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: pif1 pif3 pif4 pif5 (pif1345 mutant)
|
| Treatment protocol |
n/a
|
| Growth protocol |
Seedlings were grown in 100 µE continuous white light on 1X MS medium supplemented with 1% sucrose for 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Machery-Nagel Nucleo Spin RNA Plant Kit.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was prepared from 0.5 µg total RNA
|
| |
|
| Hybridization protocol |
The probes were hybridized in rotating hybridization chambers (Agilent Technologies) and incubated at 65 °C for 17 h overnight in a hybridization oven. Subsequently, the arrays were washed according to manufacturer’s instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
Gene expression of whole seedlings at day 5
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.5.1.1. (Agilent) using default parameters (protocol GE1-v5_10_Apr08 and Grid: 021169_D_F_20090824) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Apr 08, 2010 |
| Last update date |
Aug 23, 2010 |
| Contact name |
Claus Schwechheimer |
| E-mail(s) |
claus.schwechheimer@wzw.tum.de
|
| Phone |
08161 712880
|
| Fax |
08161 712886
|
| Organization name |
Claus Schwechheimer
|
| Street address |
WZW - Lehrstuhl Systembiologie der Pflanzen, Am Hochanger 4
|
| City |
Freising-Weihenstephan |
| ZIP/Postal code |
85354 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE21256 |
Comparison of GNC and GNL Overexpression with ga1 and pif1345 |
|
