|
| Status |
Public on Apr 14, 2010 |
| Title |
jos0672 |
| Sample type |
RNA |
| |
|
| Source name |
Replicate from 1 patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
disease state: replicate control
family history: yes
sex: male
age (years): 26
bmi (kg/m2): 59.4
fpg (mg/dl): 306
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15ug of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix GeneChip HuGeneFL array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner G2500.
|
| Description |
Replicate of gene expression from skeletal muscle of one subject without diabetes
|
| Data processing |
Data were analyzed using Affymetrix MAS 4.0, and normalized for overall intensity using linear regression.
|
| |
|
| Submission date |
Apr 14, 2010 |
| Last update date |
Apr 14, 2010 |
| Contact name |
Mary Elizabeth Patti |
| E-mail(s) |
mary.elizabeth.patti@joslin.harvard.edu
|
| Phone |
6177351966
|
| Fax |
6177322593
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Research Division
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL80 |
| Series (1) |
| GSE21340 |
Human skeletal muscle - type 2 diabetes and family history positive individuals - Mexican American |
|
