|
| Status |
Public on Jun 05, 2021 |
| Title |
Fetus |
| Sample type |
genomic |
| |
|
| Source name |
Amniotic fluid (fetus)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Amniotic fluid
|
| Treatment protocol |
Genomic DNA were extracted from amniotic fluid and peripheral blood without treatment.
|
| Growth protocol |
Amniotic fluid and peripheral blood were collected without growth.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction of amniotic fluid was conducted according to the manufacturer’s standard procedure of TIANamp Micro DNA Kit. Genomic DNA extraction of peripheral blood was conducted according to the manufacturer’s standard procedure of QIAamp DNA Blood Mini Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol from 250 ng of total DNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, more than 13.5 ug of DNA were hybridized for 16 to 18 hrs at 50 °C and 60 rpm in the GeneChip® Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned on the GeneChip Scanner 3000 as described in the CytoScan Assay User Manual.
|
| Description |
Chromosome microarray data of the amniotic fluid from a pregnant woman at 27+3 weeks of gestation by ultrasound-guided amniocentesis.
|
| Data processing |
The data were analyzed with Chromosome Analysis Suite version 4.0 using Affymetrix default analysis settings and global scaling as normalization method. Data analysis was referenced by CytoScan750K_Array.na33.r3.REF_MODEL.
|
| |
|
| Submission date |
Jun 04, 2021 |
| Last update date |
Jun 05, 2021 |
| Contact name |
Chunyan Jin |
| E-mail(s) |
gta5857@outlook.com
|
| Organization name |
Taizhou People's Hospital
|
| Street address |
399 Hailin South Road
|
| City |
Taizhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
225300 |
| Country |
China |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE176138 |
A prenatal diagnosis case of partial duplication 21q21.1-q21.2 with normal phenotype maternally inherited |
|
