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Status
Public on Apr 29, 2010
Title
NKT.44+NK1.1+.Th#2
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenize, stain MACS, double sort (Aria)
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.44+NK1.1+.Th#2, Developing thymic iNKT cells, stage 3
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 21, 2021
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE73864 Reanalyzed by
GSE180556
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.5829
10344622
289.1821
10344624
438.1569
10344633
860.1921
10344637
233.157
10344653
15.3988
10344658
165.4673
10344674
12.7018
10344679
42.048
10344707
253.6935
10344713
466.7026
10344723
297.4633
10344725
40.8975
10344741
3367.592
10344743
26.132
10344750
92.5741
10344772
25.5972
10344789
146.5756
10344797
346.8524
10344799
177.464
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538333_EA07068_91104_MoGene_NKT.44+NK1.1+.TH#2.CEL.gz
4.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
