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| Status |
Public on Jun 28, 2022 |
| Title |
Expression data of murine T-ALL OT-1 (Tot-1si) with early expression of mature alphabeta TCR |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Third-party reanalysis
|
| Summary |
In mice and humans, an immature CD4-CD8- double negative (DN) thymocyte subset expresses early, mature αβTCR. These early αβTCR+ DN (EADN) cells are susceptible to leukemogenesis in both species. Using the OT-1 TCR transgenic system to model EADN-stage αβ TCR expression, we found that EADN cells show coreceptor-independent reactivity to MHC, and their TCR:MHC dependent signals can drive T-ALL transformation. We generated gene expression data from OT-1 leukemic cells and assessed their developmental stage by comparing to wild-type thymocyte subsets.
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| Overall design |
CD4-CD8- OT-1 TCR+ leukemic cells (Tot-1si cells) were adapted to in vitro culture from the leukemic spleen of a T-ALL tumor bearing OT-1 mouse. Total RNA was extracted from cultured cells and gene expression profiling was done using Affymetrix Mouse Gene 1.0 ST Array. Tot-1si microarray data were analyzed with data of 30 wild-type thymocyte subsets from ImmGen (GSE15907). All raw datasets were preprocessed together for background correction and RMA normalization.
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| Contributor(s) |
Laffey KG |
| Citation(s) |
35767640 |
| Submission date |
Jul 21, 2021 |
| Last update date |
Jul 20, 2022 |
| Contact name |
Kimberly G Laffey |
| E-mail(s) |
laffeyk@health.missouri.edu
|
| Organization name |
University of Missouri
|
| Department |
Molecular Microbiology and Immunology
|
| Lab |
NextGen Precision Health Building
|
| Street address |
1030 Hitt Street
|
| City |
Columbia |
| State/province |
Missouri |
| ZIP/Postal code |
65211 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (1) |
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| Relations |
| Reanalysis of |
GSM854335 |
| Reanalysis of |
GSM854336 |
| Reanalysis of |
GSM854337 |
| Reanalysis of |
GSM791136 |
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GSM791137 |
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GSM791138 |
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GSM791146 |
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GSM791147 |
| Reanalysis of |
GSM791148 |
| Reanalysis of |
GSM791149 |
| Reanalysis of |
GSM791150 |
| Reanalysis of |
GSM791151 |
| Reanalysis of |
GSM791154 |
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GSM791155 |
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GSM791156 |
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GSM399403 |
| Reanalysis of |
GSM399404 |
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GSM399405 |
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GSM399391 |
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GSM399392 |
| Reanalysis of |
GSM399393 |
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GSM399379 |
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GSM399380 |
| Reanalysis of |
GSM399381 |
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GSM538332 |
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GSM538333 |
| Reanalysis of |
GSM538334 |
| Reanalysis of |
GSM476675 |
| Reanalysis of |
GSM476676 |
| Reanalysis of |
GSM476677 |
| BioProject |
PRJNA748701 |
