Heparin was injected into the jugular 5 minutes before sacrifice; right lung was flushed with PBS immediately after sacrifice, removed, and immediately converted to RNA using Qiagen rnEasy columns
Label
Biotin
Label protocol
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
Hybridization protocol
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
Scan protocol
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
Description
Pooled RNA from whole lung homogenate from three female FVB/N mouse heterozygous for Rosa26-rtTA2 transgene, fed doxycycline for 6 weeks starting at average age 5 months. Mice had near normal right ventricular systolic pressure (RVSP) at time of sacrifice. The Rosa26-rtTA2 transgene drives universal expression of a transactivator, which has no phenotype in the absence of other transgenes - this is thus a control group.
Data processing
Raw Affymetrix CHP and CEL files. Data processed using MAS5.0.
