|
Status |
Public on Aug 25, 2010 |
Title |
Control mouse + chronic ACE2 lung (1) |
Sample type |
RNA |
|
|
Source name |
Whole lung homogenate, +ACE2, -BMPR2
|
Organism |
Mus musculus |
Characteristics |
strain: FVB/N gender: female tissue: whole lung genotype: heterozygous for Rosa26-rtTA2 transgene treatment: ACE2
|
Extracted molecule |
total RNA |
Extraction protocol |
Heparin was injected into the jugular 5 minutes before sacrifice; right lung was flushed with PBS immediately after sacrifice, removed, and immediately converted to RNA using Qiagen rnEasy columns
|
Label |
Biotin
|
Label protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
|
|
Hybridization protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
Scan protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
Description |
Pooled RNA from whole lung homogenate from three female FVB/N mouse heterozygous for Rosa26-rtTA2 transgene, fed doxycycline for 6 weeks starting at average age 5 months, with recombinant human ACE2 in osmotic pumps infused for the final 2 weeks. Mice had near normal right ventricular systolic pressure (RVSP) at time of sacrifice.
|
Data processing |
Raw Affymetrix CHP and CEL files. Data processed using MAS5.0.
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|
|
Submission date |
Apr 28, 2010 |
Last update date |
May 04, 2010 |
Contact name |
James West |
E-mail(s) |
j.west@vanderbilt.edu
|
Organization name |
Vanderbilt University
|
Department |
Medicine
|
Lab |
Pulmonary
|
Street address |
4200 E 9TH Ave, Box B133
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37201 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE21583 |
Effects of ACE2 on BMPR2 mutation-mediated defects in gene expression |
|