|
| Status |
Public on Aug 25, 2010 |
| Title |
Rosa26-BMPR2-R899X mouse + chronic ACE2 lung (2) |
| Sample type |
RNA |
| |
|
| Source name |
Whole lung homogenate, +ACE2, +BMPR2
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N
gender: female
tissue: whole lung
genotype: heterozygous for Rosa26-rtTA2 and TetO7-Bmpr2-R899X transgenes
treatment: ACE2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heparin was injected into the jugular 5 minutes before sacrifice; right lung was flushed with PBS immediately after sacrifice, removed, and immediately converted to RNA using Qiagen rnEasy columns
|
| Label |
Biotin
|
| Label protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
| |
|
| Hybridization protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
| Scan protocol |
Standard Affymteric protocol (Affymetrix GeneChip Expression Analysis Manual).
|
| Description |
Pooled RNA from whole lung homogenate from three female FVB/N mouse heterozygous for Rosa26-rtTA2 and TetO7-Bmpr2-R899X transgenes, fed doxycycline for 6 weeks starting at average age 5 months, with recombinant human ACE2 in osmotic pumps infused for the final 2 weeks. Mice had near normal right ventricular systolic pressure (RVSP) at time of sacrifice. These transgenics often develop pulmonary arterial hypertension.
|
| Data processing |
Raw Affymetrix CHP and CEL files. Data processed using MAS5.0.
|
| |
|
| Submission date |
Apr 28, 2010 |
| Last update date |
May 04, 2010 |
| Contact name |
James West |
| E-mail(s) |
j.west@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Pulmonary
|
| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21583 |
Effects of ACE2 on BMPR2 mutation-mediated defects in gene expression |
|
