Fusarium fujikuroi wild type IMI58289 and Ffvel1 mutant strains were grown in liquid ICI (Imperial Chemical Industries Ltd., UK) media containing 6 mM glutamine (10 % ICI medium).
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted using the RNAgents total RNA isolation kit (Promega GmbH, Mannheim, Germany).
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
Description
Wild-type
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time.
