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| Status |
Public on Dec 31, 2010 |
| Title |
Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time. |
| Platform organism |
Fusarium verticillioides |
| Sample organism |
Fusarium fujikuroi |
| Experiment type |
Expression profiling by array
|
| Summary |
Investigation of whole genome gene expression level differences of Fusarium fujikuroi between wild-type and a Ffvel1 (velvet) deletion mutant in liquid medium with minimal nitrogen between 24 hr, 72 hr and 120 hr of growth using an array based on a F. verticillioides gene call set.
Fusarium fujikuroi produces a number of secondary metabolites including gibberellins, bikaverin, fumonisin and fusarin C that are influenced by nitrogen availability and the velvet global regulatory complex.
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| |
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| Overall design |
A twelve chip study using total RNA recovered from six cultures of wild-type Fusarium fujikuroi and six cultures of Ffvel1 F. fujikuroi deletion mutant. Each chip measures the expression level of over 13,000 putative genes with twelve 60-mer probes per sequence.
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| |
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| Contributor(s) |
Brown DW |
| Citation(s) |
20572938 |
| |
| Submission date |
May 02, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Daren W. Brown |
| E-mail(s) |
Daren.Brown@usda.gov
|
| Phone |
309-681-6230
|
| Organization name |
USDA-ARS
|
| Department |
Bacterial Foodborn Pathogens and Mycology Research
|
| Street address |
1815 N. University St.
|
| City |
Peoria |
| State/province |
IL |
| ZIP/Postal code |
61604 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8781 |
NimbleGen-Fusarium verticillioides-13K genes-60-mer-v1 |
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| Samples (12)
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|
| Relations |
| BioProject |
PRJNA127113 |
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